Consumption of 2.5% and 5% green tea extract (1ml/100g body weight) for 26days resulted in impaired spermatogenesis through the loss of matured and elongated spermatids. Oral administration of aqueous extract of green tea (70mg/kg/day) to rats for 63days did not affect weights of the body and reproductive organs, sperm concentration and viability and diameter of the seminiferous tubule33. Male Wistar rats sourced from the animal facility of the Medical Bioscience department, University of the Western Cape, had free access to standard rat chow and tap water ad libitum. You are using a browser version with limited support for CSS. . 20 Fertility Myths and Facts About Getting Pregnant - What to Expect Stress leads to increased levels of the hormone glucocorticoid, which can have a negative effect on the production of testosterone and sperm. Chances are, you won't have to give up your green tea habit if you drink in moderation. Frana, L. & Russell, L. The testis of domestic animals. Bansal, A. K. & Bilaspuri, G. S. Impacts of oxidative stress and antioxidants on semen functions. They found that excess consumption of green tea can leave a toxic effect on the body, and that can prevent in conceiving a baby. The animals were kept under standard conditions in a room at a temperature of 2124C with a constant 12h light/dark cycle. Epigallocatechin-3-gallate is absorbed but extensively glucuronidated following oral administration to mice. 9 things your guy is doing that are lowering his sperm count According to a 2017 study by the Hebrew University of Jerusalem, sperm counts among Western men have dropped more than 50 percent in the past 40 years. Green tea polyphenols inhibit testosterone production in rat Leydig cells. 48, 409416 (2010). Hence, the effect of green tea or its polyphenolic compounds on fertility rate must be further investigated to understand better the implication of spontaneous acrosome reaction observed in this study. 39, 144 (2012). 44, 265272 (2000). ISSN 2045-2322 (online). The study demonstrated a progressive rise in the weekly weight of animals, with no obvious signs of clinical toxicity or behavioural changes in the rats, indicating the well-being of the animals during the treatment period. In normal cell biology, ROS levels increase with a decreasing antioxidant response, resulting in oxidative stress which threatens sperm biology. When consumed in high doses, alcohol can affect the ability of the testes to produce sperm. Levels of ALT and AST in serum was determined93 and expressed as IU/l. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. (A) Control; (B) 2% Green tea; (C) 5% Green tea. 16, 6166 (2011). Tea association of the USA, Inc. Retrieved January 24, 2017 (2015). B. Mazza, G., Fukumoto, L., Delaquis, P., Girard, B. Briefly, 4-(Dimethylamino)-cinnamaldehyde (1,000l) was added to blank, standards and tea extract (200l) and incubated at 37C for 5min, with absorbance read at 640nm. Urol. conducted the experiments, performed the statistical analysis and drafted the manuscript. That said, moderate consumption of red wine (14 drinks or less per week, and not more than two or three at a time) may actually have a protective effect on sperm. Table 6 shows the results on lipid peroxidation and antioxidant enzymes in the testes, liver and kidney following treatment with green tea (2% and 5%). (A) Control; (B) 2% Green tea; (C) 5% Green tea. 105, 328331 (1984). We think that nutrients like folic acid on the male side may influence how complex conditions like cardiac disease, diabetes, autism, mood disorders and maybe even cancers are inherited, says Baratz. Antioxidant activities in the serum were measured using FRAP as described earlier89 and expressed as M Trolox/ ml of serum. Another study demonstrated the aphrodisiac property of black tea, accompanied by an increase in testosterone production in rats76. Previously frozen testis (20% w/v), kidney (20% w/v), and liver (20% w/v) tissues were swiftly thawed and homogenised with PRO 200 homogeniser (Proscientific Inc, Oxford, USA), in 1ml ice-cold Tris-buffered saline (TrisHCl 20mM, NaCl 150mM, pH 7.4) and centrifuged (5,000g, 30min and 4C). Yassa et al. Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice. We hate to hurt their egos, but modern mens sperm are coming up short. Toxicol. Even slightly overweight men with a BMI of 25 can have a 22 percent lower sperm count. If you must work with toxins, do so . We found that green tea negatively impacted male fertility as shown by a reduced number of offspring produced and increased mating latency. Left cauda epididymis was excised and used for the determination of sperm concentration, vitality, motility and acrosome reaction. Each group received the respective treatment ad libitum using a water bottle for 52days: Group one: tap water and served as control. Awoniyi, D. O., Aboua, Y. G., Marnewick, J. L., Plesis, S. Du & Brooks, N. L. Protective effects of rooibos (Aspalathus linearis), green tea (Camellia sinensis) and commercial supplements on testicular tissue of oxidative stressinduced rats. Shabtay, O. How Over Consumption Of Green Tea Affects Fertility In line with the observed level of testosterone and similar to a previous study33, intake of green tea in this study did not alter the weight of testosterone dependent organs (testis, epididymis, seminal vesicles and prostate gland). Effect of Soursop (Annona muricata L.) fruit extract on sperm toxicity induced by caffeine in albino rats. Am. Sci Rep 10, 15269 (2020). Ellman, G. L. Tissue sulfhydryl groups. Jimenez-Saenz, M. & del Carmen Martinez-Sanchez, M. Acute hepatitis associated with the use of green tea infusions. High levels of serum aspartate transaminase (AST), alanine transaminase (ALT), and creatinine, respectively indicate liver and renal toxicity or injury as ALT and AST are biomarkers of the liver structure and function, while creatinine is a reliable renal biomarker21,22. There are many green tea and fertility success stories. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. (a) Sperm concentration, (b) sperm vitality, (c) total motility, (d) progressive motility, (e) total static sperm, (f) acrosome reacted spermatozoa. Therapeutic Role of Green Tea Polyphenols in Improving Fertility: A Review Sperm cells are highly sensitive to reactive oxygen species (ROS), which are produced during cellular oxidation. Lipid peroxidation was assessed according to Yagi94 by measuring malondialdehyde (MDA), a thiobarbituric acid reacted substances (TBARS) and represented as nanomolar of MDA per milligram of protein. Male infertility - Symptoms and causes - Mayo Clinic The antioxidants are also classified as enzymatic (natural) oxidants (includes glutathione reductase, catalase, superoxide dismutase) and non-enzymatic oxidants, (includes vitamins B, C and E, carotenoid, carnitine, cysteine), which are derived from fruits and vegetables83. Eur. Hepatoprotective effects of green tea and its polyphenols: preclinical observations. Anabolic steroids and other illicit drugs can have the same effect. Furthermore, intraperitoneal injection of EGCG (85mg/kg body weight) for 27days caused acute body weight loss, and reduction in weight of prostate gland and testis as well as the level of testosterone and LH37. J. Nutr. If you are someone (especially a woman) who is actively trying to conceive, you will be very aware of the importance of cervical mucus in the fertilization process. Male Wistar rats were administered 2 and 5% aqueous extract of green tea for 52days ad libitum, while the control group received tap water. Excess acid can lead to digestive issues including constipation, acid reflux, and nausea. 7, e23e29 (2012). Mazzanti et al. Andrologia 41, 229234 (2009). Bar: 100m. Toxins and contaminants make their way from the lungs through the circulatory system to the testicles, says Baratz. Besides, the weight of the liver remained unchanged, with significantly reduced levels of AST and ALT. J. Nutr. 11 Harmful Green Tea Side Effects (Read Before Drinking) - Sencha Tea Bar C.S.O. During the period of treatment, at least three samples of prepared green tea (2% and 5%) were randomly collected at different time points and stored at 20C for further use. Although the beneficial effect of consumption of green tea on the overall risk of cancer remains unproven as findings from epidemiological studies revealed inconsistent results11. Food Res. Rep. 2, 252260 (2015). Absorbance was read at 450nm, and the result expressed as nanogram per millilitre. These are suggestive of possible effects of the antioxidants in these plants on various aspects of male reproductive functions. Kim, S. et al. Green tea is a popularly consumed beverage worldwide, especially in China, Japan and other Asian countries, including India5. However, a positive trend in the level of testosterone was observed in a dose-dependent manner (ANOVA-trend analysis, repeated measure: P=0.029). J. Agric. Anal. The soluble solid content (P<0.0001) as well as the ferric reducing power (P<0.0001) was also higher in the 5% in green tea extract compared to 2% green tea extract) (Table 1). Food Chem. Green tea is a popularly consumed beverage worldwide and contains polyphenols, whose antioxidant activities could improve sperm parameters and fertility thereof. Rusak, G., Komes, D., Liki, S., Hori, D. & Kova, M. Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used. Nat. 11, 362 (2009). improves sperm mobility. Dev. SpringerPlus 2, 541 (2013). Green tea consumption could be beneficial for risk reduction in cardiovascular diseases, obesity, diabetes, neurodegenerative diseases and cancer as well as weight loss9,10. Illnesses, injuries, chronic health problems, lifestyle choices and other factors may contribute to male infertility. We further identified that the lifespan extension properties of green tea was only observed in the presence of females which alludes to a reproductive (or mating) dependent mechanism. Long considered an important part of female prenatal health, folic acid is now being investigated for its ability to augment sperm quality. Biochem. Singleton, V. L. & Rossi, J. rather reported that acrosin-disrupted spermatozoa equally pierced the zona pellucida as the wild-type spermatozoa, rather, a delayed dispersal rate of cumulus oophorus cells caused a decrease in the number of fertilised oocyte and litter size74. Larson, J. L. & Miller, D. J. 7, 178184 (2015). epicatechin (EC), epigallocatechin (EGC), epicatechin-3-gallate (ECG) and epigallocatechin-3-gallate (EGCG)), with EGCG being the most abundant and effective10,12. Chinyerum Opuwari. Nutr. CAS et al. Ratnasooriya, W. D. & Fernando, T. S. P. Effect of black tea brew of Camellia sinensis on sexual competence of male rats. 15 Best Benefits of Green Tea for Fertility Health 50, 176187 (2006). Does Caffeine Affect Fertility? - Verywell Family Green tea is known to be a healthy beverage and is rich in antioxidants. The health benefits of green tea are attributed mostly to the polyphenolic compounds especially the catechins (e.g. Provided by the Springer Nature SharedIt content-sharing initiative. Philos. & Boring, D. Green tea extract-induced lethal toxicity in fasted but not in nonfasted dogs. Kaur, R. & Kaur, K. Effects of dietary selenium (SE) on morphology of testis and cauda epididymis in rats. [10] Zaminpeyma, M., & Kouchesfehani, D. (2014). Certain medications can lead to erectile dysfunction and affect the amount of ejaculate, says Dunne. Food Chem. Green tea contains caffeine, a stimulant that boosts mood, energy levels, reaction time, and memory. 13, 6771 (2013). Zidan, N. A. Kao et al. Effect of consumption of aqueous extract of green tea for 52days on the liver of male Wistar rats. Some people believe that green tea can help improve fertility and increase the chances of conceiving. J. Med. Mol. Take D-aspartic acid supplements. Data analysis was completed with GraphPad Prism version 6.0.0 for Windows (GraphPad Software, www.graphpad.com). Sharangi, A. Mahmoud, M. F., Fahmy, A. Blood obtained through a cardiac puncture, allowed to clot for 30min and centrifuged (3,000g, 15min), was stored at 80C for hormonal and biochemical assays. Tea And Pregnancy | babyMed.com Am. The L-theanine in green tea has been found to have a relaxing effect, reduce anxiety, and increase focus and concentration . Most of the beneficial effects of green tea are attributed to the antioxidant and free-radical scavenging properties, its polyphenols and flavonols80. in Male reproduction, a multidisciplinary overview 197219 (1998). The good news is that, most of the time, those levels arent permanent. Green tea also improves metabolism and weight loss. Clin. We express our appreciation to Gerhard van der Horst and Liana Maree for their assistance with the Computer Assisted Sperm Analysis equipment. 10 Ways to Boost Male Fertility and Increase Sperm Count - Healthline Lambert, J. D. et al. Scientific Reports (Sci Rep) The Scoop on Fertility Tea: Does It Really Work? | Ro Toxicology 328, 1220 (2015). Javaid, A. Updated on August 5, 2021 What is Tea? Stutler, S. A. et al. excess caffeine could have an adverse effect. Dose-dependent effects of caffeine in human Sertoli cells metabolism and oxidative profile: relevance for male fertility. Mazzanti, G., Di Sotto, A. After that, contents were briskly shaken out and rinsed three times with diluted wash solution (400l) and stricken sharply on absorbent paper to remove residual droplet. & M., Tamanna, N. & Haque, Md. Similarly, GSH, CAT and SOD levels in the kidney, liver and testes remained unchanged (P>0.05). 55, 874885 (2011). Effects of repeated green tea catechin administration on human cytochrome P450 activity. Conversely, other contributions suggested a negative effect of cola-containing beverages and caffeine-containing soft drinks on semen volume, count and concentration. In brief, standard solution (0, 15, 30, 60, 90, 120 and 150mg/l creatinine in dH20) or serum was added to a working solution (0.1g picric acid in 50ml dH20 and 50ml 0.4 NaOH in 4C) (1:10), incubated at RT for 15min and measured at 492nm. Effect of green tea (Camellia sinensis L.) extract on morphological and functional changes in adult male gonads of albino rats.